Earthern and Pot Culture Method to Check the Stability of Marine Azotobacter in Soil

A pot culture experiment was conducted by C.V Kanchan to evaluvating the effects of Azotobacter inoculants on the yield of wheat. M.A kader(2002) was conducted a pot culture experiment on straw. He found significant increase in root growth by the treatment of Azotobacter .

S.K.Kavimandan (1986) was carried out a pot experiment with an Azotobacter chroococcum along with 50 Kg N /Ha. He found an adverse effect of bacterial inoculation on the yield of wheat. Choudhury .A(2005) carried out pot culture experiment on three rice cultivars with eight different N2 fixing bacteria strains with the objective to find out effective nitrogen fixer. He found that Azospirillum appeared to be the best followed by Pseudomonas and Azotobacter when inoculated to rice variety. Ravikumar et al (2004) found inoculation with Azotobacter in mangrove soil increase seedlings,. Root biomass, shoot biomass ,total chlorophyll of plant . thus azotobacterisation is beneficial in raising vigorous seedlings of mangrove in coastal wetlands.

CONCLUTION :

A marine sample indicates that the concentration of nitrogen-fixing organisms is much lower in oceanic environments than in coastal environments. However, even at low densities, active population of nitrogen- fixing microorganism over vast areas of the open ocean could contribute substantially to the nitrogen inputs in the world’s ocean (Zehr et al 1998)

This study revealed that marine Azotobacter can be cultivated in laboratory condition, which provides more information on growth pattern on different media. Water analysis result showed high concentration of calcium, magnesium, chloride content.

Acetylene reduction assay was performed and checked the enzyme activity of randomly selected samples and were used for pot culture experiment. The pot culture experiment showed significant increase in shoot, root length of the plant. Hence marine Azotobacter can survive in soil and fix atmospheric nitrogen. Marine Azotobacter can be used as a suitable biofertilizer in order to reduce the usage of chemical fertilizer which is potent harmful substances mainly petrochemicals.

LITERATURE CITED:

Bedford, R.H, 1933. Marine bacteria of the northern pacific ocean. The temperature range of growth. Contrib. can. Bio. Fisheries 8: 433-438.

Burk, D., and Horner, C.K. 1940. Molybdenum and calcium in Azotobacter nutrition. Proe. Third Intern. Congr. Microbiol. (New York), p 489-490.

CHOUDHURY . A 2005. Screening of rice cultivars and diazotrops combination for better N(2) fixing system. Indian journal of plant physiology Vol 10 p 82-85

Eisenstarh A., K.J. McMahon and Roma Eisenstarh, 1949. Department of Bacteriology, Oklahoma. A cytological study of pleomorphic strain of Azotobacter with the electron and phase Microscope and the Robinson Nuclear – Staining Technique.

Guerinot, M.L., and Patriquin D.G. 1981. The association of N2-fixing bacteria with sea urchins. Mar. Biol. Vol 62: 197-207.

Hans W.Parel. Microbially Mediated Nitrogen Cycling. Techniques in Microbial Ecology, P 4-27 – ND.

Hardy R.W.F. 1968. Acetylene ethylene assay for nitrogen fixation: Laboratory and field evaluation. Plant physiology vol.43, 1185-1207.

Hegazi, M. Monib and Vlassak K, 1978. Effect of inoculation with N2-Fixing Spirilla and Azotobacter on Nitrogenase Activity on Roots of Maize Grown Under subtropical conditions, vol.38 No.4. P 621-625.

James. A. Coyer, Alejandrocabello – Pasini, Hewson Swift and Randall. S. Alberte, 1996. N2 fixation in marine hetrotrophic bacteria dynamic of environmental and molecular regulation. Vol 93: P 3575 – 3580.

Jensen. H.L. 1954, The Azotobacteriaceae. Bacteriological Rev. 18: 195-214.

S.K Kavimandan 1986 Influence of rhizobia,azotobacter and blue green algae on n content and yield of rice .Vol 96 133-135

M.A Kader 2002. Effects of Azotobacter inoculant on the yield and nitrogen uptake by wheat ,Journal of biological sciences , vol 2(4) p 259-261

Lewis I.M. 1937. Cell inclusions and life cycle of Azobacter J. Bacteriol 34: 191-205.

MacLeod. R.A. and Onofrey, 1957. Nutrition and metabolism of marine bacteria III. The relation of sodium and potassium to growth J. Cell. Comp. Physiol. Vol 50: 398-409.

Maria GT – Rubio, Sandra AVP., Jaime Bernal – Castilo, Patrica Martinel- Nieto, 2000. Association Latinoamericana de microbiologia. Vol 42: 171-176.

Mary LG and Rita R Colwell 1985, Enumeration, isolation and characterization of N2 fixing bacteria from sea water. Department of Microbiology, University of Maryland. Vol 50 No.2.

Murray C.M.J.P. Riley: and T.R.S. Wilson 1969. The solubility of gases in distilled water and sea water I Nitrogen. Deep sea. 16: 297-310.

Page W.J. and H.L. Sadoff, 1975. Relationship Between Calcium and Uronic Acids in the Encysment of Azobacter vinelandii. Journal of Bacteriology, Vol.122, No.1 p 145-151.

Postgate, J.R (1982). Fundamentals of nitrogen fixation (Cambridge Univ. Press. Cambridge, U.K).

Rai. M.K, Handbook of Microbial Biofertilizers, an imprint of the Haworth Press, Inc. New York, London, Oxford.

Ramos J.L and R.L. Robinson, 1985. Isolation and properties of mutant of Azobacter chroococum defective in aerobic nitrogen fixation J. Gen. Microbial. 131: 1449-1458.

Ravikumar et al 2004. Nitrogen fixing Azotobacter from mangrove habitate and their utility as marine biofertilizera, journal of experimental marine biology and ecology Vol312 p5-17

Richter. O. 1928. Natrium: Ein notwendiges N’ahrelement fur eine marine mikroarophile Leuchbakterie. Anz. Oesterr. Akad. Wiss. Math. Naturw. KI. 101: 261-292.

Robert A Macleod, 1965. The question of the existence of specific Marine bacteria, department of bacteriology, American society for microbiology, McGill University, Canada. Vol.29 No.1.

Robinson G.G.C.L.L. Hendzea and D.C. Giillespie 1973. A relation between heterotrophic utilization of organic acids and bacterial population in West Blue iake, Manitoba, Limnon, Oceanogr 18: 264-269.

Tyler. M.E., M.C.Bielling and D.B. Pratt, 1960. Mineral requirements and other characters of selected marine bacteria Jou. Gen. Microbiol. Vol.23: 153-161.

Vela G.R. and Rosenthal R.S., 1972. Effect of Peplon on Azotobacter Morphology, America Society for Microbiology. Vol.111 No.1.

Zobell. C.E and Upham H.C., 1994. A list of marine bacteria including description of sixty new species. Bull. Scripps Inst. Oceanography. Vol. 5: 239-292.

Standard method for the examination of water and waste water, 16th edition, APHA, AWWA, WPCF.

* Cristian G.D. Analytical Chemistry 4th Edition, J. Wiley and Sons.

* Harris D.C. Quantitative Chemical Analysis 5th Edition, W.H. Freeman.

* History of nitrogen fixation. From: Biology 446, Uni. Of Watterloo (Biology 446).

* http://www.indiaagronet.com/

* http://www.thekrib.com/plant/co2/hardness-larryfr.,html.

* The microbial world: The nitrogen cycle and nitrogen fixation produced by Jim Deacon, Institute of cell and molecular biology, the University of Edinburgh.

* Determination of hardness of water method WHO/M/26.RI, revised 10 Dec. 1999.

* htt://www.nalms.org/

Carlson, R.E. and J. Simson, 1996. A coordinators Guide to volunteer lake Monitoring Methods, North American lake Management Society, P 96.

* Determination of water hardness by EDTA Titration from Gannon University SIM.

* http:/www.bact.wisc.edu/The world of Microbes.htm

*web.centre.edu/shiba/che117L/exp8_hardness.htm:

Pages from web.centre.edu.

* http://www/tau/ac/

The nitrogenous complex

* http://www.bookrags.com

Azotobacter compete article

*Shri Dorji Tenzing Bhutia, 2004. Joint Director Skims, Biofertilizers for Nutrient Management in Organic Production of Agri / Horticultural crops.

* http://cos.colstate.edu/stokes/chlorophyll.htm.

*http://www.usoe.k12.ut.us/curr/science/scriber/00/8th/energy/scriber/chlorophyll.htm.

Chlorophyll : why?

** Atlas R. and Bartha R, 1998. Microbial Ecology Fundamentals and Applications 4th Edition Benjamin Cummings. Menlopark. Ca.694pp.

** Camphell. N. 1993. Biology 3rd Edition. Benjamin Cummings, Redwood City, Ca 1190.

** N.S.Subba Rao, Soil Microbiology and plant growth 4th edition.

** Robert L. Tate, 1995. Soil Microbiology. Jhon Willey and Sons, Inc.

** Jan Dirk Van Elsas, Jack T. Trevors, Elizabeth, M.H.Willington 1997, Modern Microbiology.

**S.Sadasivam and A.Manikam, 2004. Biochemical methods, 2nd Edi., Centre for plant molecular biology, TNAU/

(* Net reference)

(** Book Reference)

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